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为了探究枣黄化根蘖苗的成因,对其黄化根蘖苗、正常根蘖苗和母株之间的形态学指标、叶绿素及矿质元素含量和相关基因表达水平进行了分析。结果表明,与正常植株比,黄化根蘖苗形态学指标没有明显变化,但叶绿素含量及叶绿素a/b比值显著降低,矿质元素N、P、K、Mn、Cu、Zn、Fe含量显著增高,但Ca和Mg差异不显著。同时,AFLP分析表明,黄化根蘖苗与正常植株在基因组水平没出现显著差异;但cDNA-AFLP和RT-PCR分析表明在转录水平上,黄化根蘖苗中酰基转移酶基因表达增加,Ty3-gypsy反转录转座子基因和GAGA结合转录激活因子基因表达降低。综合分析认为,枣根蘖苗的黄化可能是与叶绿素合成转录相关的反转录转座子和GAGA结合转录激活因子基因表达降低,影响了叶绿素合成,使植株最终表现黄化;同时黄化植株中与光呼吸相关的一些基因,如酰基转移酶基因表达相应增加以适应这种逆境胁迫,说明这些基因间存在着相互适应的调控网络。
In order to explore the causes of etiolated tillering seedlings, the morphological indexes, contents of chlorophyll and mineral elements and the expression of related genes in etiolated tiller seedling, normal tillering seedling and parental plant were analyzed. The results showed that compared with normal plants, the morphological indexes of yellowtail root tillers did not change significantly, but the content of chlorophyll and the ratio of chlorophyll a / b decreased significantly, while the content of mineral elements such as N, P, K, Mn, Cu, Zn and Fe increased significantly There was no significant difference between Ca and Mg. At the same time, AFLP analysis showed that there was no significant difference in genomic level between the tillering plants and the normal plants. However, cDNA-AFLP and RT-PCR analysis showed that at the transcriptional level, the expression of acyltransferase gene in the yellow root tillers increased, Transcriptional transposon genes and GAGA-binding transcriptional activator gene expression decreased. The results showed that yellowing of Zanthoxylum tilleriae may be related to the reduction of chlorophyll transcription and transgene expression of GAGA-binding transcriptional activator, affecting chlorophyll biosynthesis and eventually yellowing of plants. At the same time, Some plant photorespiration-related genes, such as acyltransferase gene expression increased to adapt to this stress, indicating that there is a mutual adaptation of these genes regulatory network.