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目的探讨磷脂氢谷胱甘肽过氧化物酶(PHGPx)在p,p’-DDE对大鼠睾丸生精细胞脂质过氧化及诱导生精细胞凋亡中的作用。方法将20只健康清洁级50日龄雄性SD大鼠按体重随机分为4组,分别为对照(玉米油)组及低(20 mg/kg)、中(60 mg/kg)、高(100 mg/kg)剂量p,p’-DDE染毒组,每组5只。采用腹腔注射方式染毒,注射容积为10ml/kg,每隔1 d染毒1次,连续进行10 d。测定血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)的含量。采用Western blotting方法和检测大鼠睾丸组织PHGPx蛋白相对表达情况,采用实时荧光定量PCR(FQ-PCR)测定大鼠睾丸组织PHGPx及B细胞淋巴瘤-2相关X蛋白(Bcl-2 associated protein X,Bax)、细胞色素C(Cyt C)、凋亡蛋白酶活化因子-1(Apaf-1)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)mRNA的表达水平。结果与对照组相比,60 mg/kg p,p’-DDE染毒组血清SOD、GSH-Px活力和睾丸组织PHGPx蛋白表达水平均降低,100 mg/kg p,p’-DDE染毒组大鼠睾丸组织中PHGPx、Cyt C、Apaf-1 mRNA表达水平和60 mg/kg p,p’-DDE染毒组Caspase-3 mRNA表达水平较高,60 mg/kg、100 mg/kg p,p’-DDE染毒组Bax mRNA表达水平均升高,差异有统计学意义(P<0.05)。结论PHGPx在p,p’-DDE所致男(雄)性生殖功能紊乱中起拮抗作用。
Objective To investigate the effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) on lipid peroxidation of rat spermatogenic cells and apoptosis of spermatogenic cells induced by p, p’-DDE. Methods Twenty healthy male Sprague-Dawley (SD) rats at 50 days of age were randomly divided into 4 groups according to body weight: control (corn oil) group and low (20 mg / kg) mg / kg) dose p, p’-DDE exposure group, 5 rats in each group. The rats were injected intraperitoneally with an injection volume of 10 ml / kg once a day for 10 days. The activities of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were determined. Western blotting was used to detect the relative expression of PHGPx protein in testis tissue of rats. Real-time quantitative PCR (FQ-PCR) was used to detect the expression of PHGPx and Bcl-2 associated protein X Bax, Cyt C, Apaf-1 and Caspase-3 mRNA were determined. Results Compared with the control group, the activities of SOD, GSH-Px and PHGPx in the serum of 60 mg / kg p, p’-DDE group were lower than those of the control group The mRNA expression levels of PHGPx, Cyt C and Apaf-1 in rat testis tissue were significantly higher than that in 60 mg / kg p and p’-DDE treatment groups (60 mg / kg, 100 mg / kg p, The expression of Bax mRNA in p’-DDE group was significantly higher than that in control group (P <0.05). Conclusions PHGPx plays an antagonistic role in male and female reproductive disorders induced by p, p’-DDE.