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本研究通过整体染毒后分离小鼠睾丸间质细胞或体外处理原代培养的小鼠睾丸间质细胞，评价镍对小鼠睾丸间质细胞分泌睾酮（Ｔ）的影响。（１）体内研究：选择体重３５～４５ｇ的小鼠２０只，分为４组，每组５只。分别每隔３天经皮下注射１０、２０、４０ｍｇ／ｋｇ硫酸镍（Ｎｉ
In this study, primary cultured mouse Leydig cells were isolated from the whole mouse testicular stromal cells or treated in vitro to evaluate the effect of nickel on the secretion of testosterone (T) in mouse Leydig cells. (1) In vivo study: Twenty mice weighing 35-45 g were selected and divided into 4 groups of 5 mice each. Subcutaneous injection of 10, 20 and 40 mg / kg nickel sulfate (Ni