AIM:To examine the role of nucleostemin in the growthregulation of gastric cancer,liver cancer and other cancers.METHODS:RT-PCR was used to clone the fragment ofnucleostemin cDNA from HEK 293 cells.Eighteen kinds ofmalignant tumor tissues including gastric adenocarcinomaand liver cancer tissues,3 kinds of benign tumor tissues,3kinds of benign hyperplastic tissues and normal tissueswere employed to examine nucleostemin gene expressionby RT-PCR,Slot blot,Northern blot and in situ hybridization.RESULTS:We successfully cloned a 570 bp fragment ofnucleostemin-cDNA from HEK-293 cells.All detectedmalignant tumor tissues,benign tumor tissues,and benignhyperplastic tissues had high levels of nucleosteminexpression.Nucleostemin was also expressed in humanplacenta tissue at a high level.In terminally differentiatednormal human adult kidney and mammary gland tissues,no nucleostemin expression could be detected.CONCLUSION:Nucleostemin can help regulate theproliferation of both cancer cells and stem cells.It mightplay an important role in the growth regulation of gastriccancer,liver cancer and other cancers. AIM: To examine the role of nucleostemin in the growthregulation of gastric cancer, liver cancer and other cancers. METHODS: RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues , 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissueswere employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization .RESULTS: We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK -293 cells. A detected genignant tumor tissues, benign tumor tissues, and benignhyperplastic tissues had high levels of nucleostemine expression. Nucleostemin was also expressed in humanplacenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.CONCLUSION: Nucleostemin can help regulate the proliferation of both cancer cells and stem cells.It mightplay an important role in the growth regulation of gastriccancer, liver cancer and other cancers.