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目的观察狼疮肾炎(LN)患者尿巨噬细胞移动抑制因子(MIF)浓度是否升高及其与LN肾组织MIF表达、肾脏病理及功能损害的关系,试图寻找一种反映狼疮肾损害的非创伤性指标。方法用ELISA方法测定LN患者血、尿MIF浓度。用免疫组织化学双染技术观察肾组织MIF表达及巨噬细胞浸润情况。了解尿MIF水平与狼疮肾组织MIF表达、巨噬细胞浸润、狼疮肾组织活动指数、肾脏功能及组织学损害的关系。结果LN患者肾组织MIF表达及尿MIF浓度较正常人明显增高,尤以增生及炎症明显的Ⅲ、Ⅳ型LN为明显,尿MIF浓度与肾组织MIF表达、巨噬细胞浸润、狼疮肾组织活动指数、肾小管损害显著相关,但与血MIF水平、蛋白尿程度及肾功能损害无显著相关。结论LN患者尿MIF浓度明显增高,并与肾组织MIF表达及狼疮肾组织活动情况显著相关,可作为一种监测狼疮肾活动及肾损害的非创伤性指标。
Objective To investigate the relationship between the increase of urinary macrophage migration inhibitory factor (MIF) and the expression of MIF in nephrotic tissues and the pathological and renal dysfunction of LN in patients with lupus nephritis (LN) and to find a non-invasive Sexual indicators. Methods Blood and urine MIF concentrations were measured by ELISA in patients with LN. The expression of MIF and the infiltration of macrophages in renal tissues were observed by immunohistochemical double staining. To understand the relationship between urinary MIF levels and MIF expression in lupus renal tissues, macrophage infiltration, lupus nephrotic index, renal function and histological damage. Results The expression of MIF and urinary MIF in LN patients were significantly higher than those in normal controls, especially in type Ⅲ and type Ⅳ patients with hyperplasia and inflammation. The expression of MIF and the expression of MIF, macrophage infiltration and lupus nephropathy Index, tubule damage was significantly correlated, but not with blood MIF levels, proteinuria and renal dysfunction was not significantly correlated. Conclusions The urinary MIF concentration in patients with LN is significantly higher than that in patients with LN, and is correlated with the expression of MIF in renal tissue and the activity of lupus nephropathy. It can be used as a noninvasive indicator to monitor renal activity and renal damage in lupus.