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为了比较基底前脑神经元移植至海马后胆碱乙酰转移酶、神经生长因子受体和一氧化氮合酶神经元的变化规律。选用雄性 SD大鼠 35只 ,在单侧穹隆海马伞损伤后一周 ,将胚胎基底前脑神经元植入损伤侧海马。在移植术后存活 5 d,7d,14 d,30d,6 0 d,90 d,和 180 d共分为 7个时间组灌注取材 ,进行 Ch AT和 NGFR的免疫组织化学反应及 NOS反应 ,观察其神经元的变化。结果证明 ,Ch AT神经元在移植后 30 d方出现阳性反应 ;NGFR神经元在移植后 7d可见淡染的胞体 ,随后逐渐增强 ;NOS神经元在移植后 7d时呈明显的阳性反应 ,而且数量多。三种神经元直到 180 d时均尚呈阳性反应。提示移植物内 NOS神经元出现早且数量多 ,NGFR神经元出现的也较早 ,Ch AT神经元出现最晚。这三种物质在细胞内出现的时间和数量差异与它们在细胞内发挥的功能有关
To compare the changes of choline acetyltransferase, nerve growth factor receptor and nitric oxide synthase neurons in basal forebrain neurons after transplanted to the hippocampus. Thirty-five male Sprague-Dawley rats were used to implant embryonic basal forebrain neurons into injured lateral hippocampus one week after injury of unilateral vaultal hippocampus. The survival time after transplantation was 5 days, 7 days, 14 days, 30 days, 60 days, 90 days and 180 days. The rats were divided into 7 groups for perfusion. Immunohistochemistry and NOS reaction of ChAT and NGFR were observed and observed Its neuronal changes. The results showed that Ch AT neurons showed a positive reaction on the 30th day after transplantation. NGFR neurons showed lightly stained cells 7 days after transplantation, and then gradually increased. NOS neurons showed a positive reaction at 7 days after transplantation, and the number of many. Three neurons did not show positive reaction until 180 days. These results suggest that NOS neurons in the grafts appear earlier and more frequently, NGFR neurons appear earlier and Ch AT neurons appear late. The time and amount differences of these three substances in the cells are related to their function in the cells