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目的 研究来自内南五味子的戈米辛J对脂质过氧化的影响和清除超氧阴离子自由基 (O·2 )的能力。方法 采用离体大鼠肝线粒体膜的脂质过氧化模型和黄嘌呤氧化酶 鲁米诺化学发光法。结果 戈米辛J象VitE一样能剂量依赖性抑制Fe2 + VitC和ADP/NADPH所致的脂质过氧化 ;戈米辛J的IC50 分别为 5 75 ( 95 %可信限 :5 42～ 6 11)和0 95 ( 0 14～ 6 5 4) μmol·L-1。VitE的IC50 分别为 74 8( 30 2～ 185 3)和 6 5 1( 0 13～ 319) μmol·L-1。戈米辛J抑制Fe2 + VitC和ADP/NADPH所致的脂质过氧化的作用比VitE的作用分别强 13倍和 6 8倍。戈米辛J也剂量依赖地抑制黄嘌呤 黄嘌呤氧化酶 鲁米诺化学发光 ,其抑制发光强度 5 0 %的浓度 (IC50 )为 2 18 2 2 μmol·L-1。结论 戈米辛J具有抑制OH·诱导的脂质过氧化和清除O·2 的作用
Objective To study the effect of Gomisin J on the lipid peroxidation and the ability to scavenge superoxide anion radical (O·2 ) from Schisandra chinensis. Methods Lipid peroxidation model of rat liver mitochondrial membrane and xanthine oxidase luminol chemiluminescence method were used. RESULTS: Gomisin J, like VitE, dose-dependently inhibited lipid peroxidation induced by Fe2 + VitC and ADP/NADPH; the IC50 of Gomisin J was 5 75 (95% Confidence Limit: 5 42 - 6 11). ) and 0 95 (0 14 to 6 5 4) μmol·L-1. The IC50 of VitE was 74 8 (30 2 to 185 3) and 651 (0 13 to 319) μmol·L-1, respectively. Gomissin J inhibited the lipid peroxidation induced by Fe2 + VitC and ADP/NADPH, which were 13 and 64 times more potent than that of VitE, respectively. Gomisin J also dose-dependently inhibited xanthine xanthine oxidase luminol chemiluminescence, and its concentration of inhibitory luminescence 50% (IC50) was 2182.2 μmol·L-1. Conclusion Gomisin J has the effect of inhibiting OH-induced lipid peroxidation and scavenging of O·2