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瑞典Uppsala大学Tydèn等应用羊水细胞学、肌酐、L/S比例及磷脂甘油测定四种试验来确定胎儿成熟度目的在研究:(1)用单项或联合成熟试验预测肺成熟度的价值如何?(2)羊水试验能否作为妊娠后期确定胎龄的方法? 作者从196名孕妇得262份羊水标本,其中116份是足月前(孕37周或<37周)经腹穿收集的,穿刺的指征是选择性剖腹产(狭窄骨盆、臀位)、高血压疾患、Rh同种免疫、糖尿病、宫内生长迟缓等。11份标本是在阴道自然分娩时抽取。196名孕妇中139人剖腹产,39人引产,18人自然分娩,羊水细胞学检查按Morrison法记录含脂细胞百分率,以10%代表成熟。肌酐浓度用离心标本经自动分析机测定,参考值为160μmol/L。卵磷脂/鞘磷脂比例按Gluck法测定而以比值1.8为参考值。磷脂甘油按Hallman系在二维薄层色层分析系统中测定,经碘蒸气染色确定磷脂甘油存在为成熟,应用
Tydèn Uppsala University in Sweden and other applications Amniotic fluid cytology, creatinine, L / S ratio and phospholipid glycerol determination of four tests to determine fetal maturity Objective To study: (1) single or combined with mature test predict the value of lung maturity? ( 2) Amniotic fluid test can be used as a method of determining gestational age in the second trimester? The authors obtained 262 amniotic fluid samples from 196 pregnant women, of which 116 were collected by abdomen for a full term (37 weeks or <37 weeks) Indications are selective caesarean section (narrow pelvis, breech), hypertension, Rh alloimmunity, diabetes, intrauterine growth retardation and so on. Eleven specimens were taken during spontaneous labor in the vagina. 139 pregnant women in 196 pregnant women by caesarean section, 39 induced labor, 18 natural childbirth, amniotic fluid cytology by Morrison method to record the percentage of fat-containing cells, with 10% representative of maturity. Creatinine concentration using centrifuge samples were determined by automatic analyzer, a reference value of 160μmol / L. Lecithin / sphingomyelin ratio by Gluck method to determine the ratio of 1.8 as a reference value. Phospholipid glycerol was determined by Hallman’s system in a two-dimensional thin layer chromatographic system. The presence of phospholipid glycerol was confirmed by iodine vapor staining.