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In the present study,agar diffusion test and field trials were carried out using a mushroom(Pleurotus ostreatus)cultivar Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasii and its effects on mycelial growth of mushroom.The results revealed that 1.25%,2.50%and 5.00%of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom,but also greatly promoted mushroom mycelial growth.Therefore,1.25%and 2.50%of refined pyroligneous acid can be used in fields to prevent brown blotch disease,and 5.00%refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.
In the present study, agar diffusion test and field trials were using a mushroom (Pleurotus ostreatus) cultivar Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasii and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid significantly inhibited the occurrence of brown blotch disease in mushrooms, but also substantial promoted mushroom mycelial growth.Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.