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植物名称:晚香玉(Polianthes tuberosa) 材料类别:块茎培养条件:接种培养基为MS,蔗糖浓度3％,琼脂0.7％,pH调至6.0,其附加激素类物质有:(1)NAA 1mg/l(单位下同)+ZT0.2;(2)NAA1+BAO.1;(3)BA1+NAA0.1;(4)BA1+KT1+NAA0.5。培养温度为25±2℃,每天光照10小时,光强为2支 40W日光灯。生长与分化情况:取块茎洗净后,切成0.6×0.6×0.4cm见方,接种在上述各种培养基上。经一个月培养后,在1号培养基上,光长数条根而已,却没有芽的分化;在2—4号培养基上,均有芽的分化,
Botanical name: Polianthes tuberosa Material category: tuber Culture conditions: Inoculation medium MS, sucrose concentration 3%, agar 0.7%, pH adjusted to 6.0, the additional hormones are: (1) NAA 1mg / l (unit same below) + ZT0.2; (2) NAA1 + BAO.1; (3) BA1 + NAA0.1; (4) BA1 + KT1 + NAA0.5. Culture temperature 25 ± 2 ℃, light 10 hours a day, the intensity of 2 40W fluorescent lamp. Growth and differentiation: Take tubers washed, cut into 0.6 × 0.6 × 0.4cm square, inoculated on the above various media. After a month of culture, on the No. 1 medium, only a few roots of light only, but no bud differentiation; 2-4 in the medium, bud differentiation,