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旋毛虫感染后的第2～3周是急性期症状出现的主要阶段。这一时期幼虫的生化、免疫学研究对急性期病人的早期诊断和治疗具有极重要的意义。目前,对旋毛虫的研究集中于成虫和肌肉幼虫阶段,对于引起主要临床症状的新生幼虫(NBL),则因为收集大量纯净NBL困难而研究较少。用传统方法收集NBL易污染且数量有限,我们参照高田伸弘等的方法并略加改进,使大量、高效收集纯净NBL成为可能。一、材料和方法 (一)旋毛虫成虫的收集取雄性大白鼠,每只经口感染旋毛虫肌肉幼虫10 000条。感染后第5d断食,第6d处死。无菌消毒后剖腹取出小肠,纵行剪开。大鼠
Trichinella infection 2 to 3 weeks after the onset of acute symptoms of the main stage. The biochemical and immunological studies of larvae during this period are of great importance to the early diagnosis and treatment of acute patients. Currently, studies of Trichinella concentrate on adult and muscle larval stages, and less research is focused on neonate larvae (NBL), which cause the major clinical symptoms, due to the difficulty of collecting a large amount of pure NBL. The traditional method to collect NBL is easy to pollute and the quantity is limited. We refer to Takata Takayuki and other methods and slightly improved, so that a large number of highly efficient collection of pure NBL possible. I. Materials and Methods (I) Collection of Trichinella Adult Male rats were orally infected with 10 000 muscle larvae of Trichinella. 5d after infection, died on the 6d. Aseptic disinfection Caesarean section after removal of small intestine, longitudinal cut open. Rat