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室内条件下测定了当归根际土水浸液对当归种子萌发、幼苗生长的影响,采用化感作用效应指数(RI)和综合化感效应指数(M)及差异性检验评价了当归根际土的化感效应。结果表明:与蒸馏水和对照土实验组相比,根际土、非根际土水浸液对种子萌发、幼苗生长及4种酶活性均有显著的抑制作用;随着质量浓度的升高,根际土水浸液对当归种子萌发和幼苗生长的综合化感效应在各浓度(0.125～1.000g·ml-1)之间差异显著(P<0.05),而在相同质量浓度条件下,根际土水浸液的综合化感效应较非根际土和对照土明显;当质量浓度均为0.500g·ml-1时,根际土、非根际土水浸液对4种酶活性均有明显抑制作用,且二者之间的差异达到显著水平(P<0.05);3种土壤水浸液供体对当归种子萌发、幼苗生长的抑制强度均表现为根际土>非根际土>对照土,因此推测自毒作用是当归连作障碍的原因之一。
The effects of anaerobic rhizosphere soil water extract on the germination and seedling growth of Angelica sinensis were determined under indoor conditions. The allelopathic effect index (RI) and comprehensive allelopathy index (M) and the difference test were used to evaluate the effects of angelica rhizosphere soil Allelopathy. The results showed that the rhizosphere soil and non-rhizosphere soil water extract had significant inhibitory effect on seed germination, seedling growth and four enzyme activities compared with the experimental group of distilled water and control soil. With the increase of mass concentration, The generalized allelopathic effect of rhizosphere soil aqueous extracts on the germination and seedling growth of Angelica sinensis had significant difference (P <0.05) at each concentration (0.125-1.000g · ml-1), but under the same mass concentration, The comprehensive allelopathic effects of the intercalary aqueous extracts were more obvious than that of the non-rhizosphere soils and the control soils. When the concentrations were both 0.500g · ml-1, (P <0.05). The inhibitory strength of three kinds of soil aqueous extracts on the germination and seedling growth of Angelica sinensis were both rhizosphere soil> non-rhizosphere soil > Control soil, it is speculated that autotoxic effect is one of the reasons Angelica continuous cropping obstacles.