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目的了解中国HIV/AIDS患者tat第一外显子的基因序列的特征和变异特点,探讨其与HIV-1感染者疾病是展之间的关系。方法选取40例辽宁和吉林HIV感染后临床进程不同的人抽取外周血,提取核酸,用套式聚合酶链反应(PCR)扩增HIV-1的tat基因,并进行核酸测序,生成系统进化树。结果长期不进展者、无症状感染者及AIDS患者的tat基因序列与相应亚型的国际流行株序列比较,各组均存在与流行株序列不一致的氨基酸变异,而且某些变异具有组间特异性。在系统树分析中各组聚集在一起,呈混合分布。结论中国HIV/AIDS患者tat第一外显子一些位点的基因变异可能与疾病进展有关,某些位点的基因变异可能会降低病毒的复制能力。
Objective To understand the characteristics and variation of the gene sequence of tat exon in HIV / AIDS patients in China and to explore the relationship between the disease and the HIV-1 infection. Methods Forty people with different clinical course after HIV infection in Liaoning and Jilin were enrolled in this study. Peripheral blood was collected from patients with HIV infection and nucleic acids were extracted. The tat gene of HIV-1 was amplified by nested polymerase chain reaction (PCR) and sequenced. The phylogenetic tree . Results There was no amino acid variation in the tat gene sequences of asymptomatic and AIDS patients compared with those of the corresponding subtypes in the long-term non-progression-free, asymptomatic and AIDS patients, and some of the variations were inter-group specific . In the phylogenetic tree, all the groups are clustered together and mixedly distributed. Conclusion The genetic variation of some exons of tat in Chinese HIV / AIDS patients may be related to the progress of the disease. The genetic variation in some loci may reduce the replication ability of the virus.