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目的:探讨男性泌尿生殖道不同部位标本(尿道分泌物、前列腺液、精液)解脲支原体和沙眼衣原体检测结果的差异,为临床检测标本选择提供依据.方法:采用实时荧光定量PCR技术对516例疑似泌尿生殖道感染者的尿道分泌物、前列腺液、精液标本进行解脲支原体和沙眼衣原体检测.结果:尿道分泌物标本解脲支原体和沙眼衣原阳性率分别为31.8％(164/516)和6.2％(32/516);前列腺液标本解脲支原体和沙眼衣原体感染率分别为34.1％(176/516)和7.0％(36/516);精液标本解脲支原体和沙眼衣原体感染率分别为“,”Objective: To investigate the difference of detection results of Ureaplasma urealyticum and Chlamydia trachomatis in different parts of male urogenital tract ( urethral secretion, prostatic fluid and semen), and to provide basis for clinical specimen selection. Methods: Ureaplasma urealyticum ( UU) and Chlamydia trachomatis ( Chlamydia trachomatis) were detected in 516 cases of suspected urogenital tract infection by real -time fluorescence quantitative PCR. Results: The positive rates of Ureaplasma urealyticum and Chlamydia trachomatis in urethral secretions were 31.8％ (164/516) and 6.2％ (32/516), 34. 1％ (176/516) and 7. 0％ (36/516) in prostatic fluid, 37.9％ (195/516) and 8. 3％ (43/ 516) in semen, respectively. 195 Ureaplasma urealyticum positive specimens covered 176 prostatic fluid positive specimens and 164 urethral secretions positive specimens. The missed diagnosis rate of Ureaplasma urealyticum in semen was 0, that of prostatic fluid was 9. 7％ ( 19/195 ), that of urethral secretion was 15. 8％ (31/195 ), and that of Chlamydia trachomatis in semen was covered by 36 positive samples of prostatic fluid and32positivesamplesofurethralsecretion.ThemisseddiagnosisrateofChlamydiatrachomatisinsemenwas0, thatofprostaticfluidwas16. 2％ (7/43 ), and that of urethral secretions was 25. 8％ ( 11/43 ) . Conclusions:The detection rate of wide coverage of semen samples is high. ThesemenshouldbethefirstchoiceforthedetectionofUreaplasmaurealyticum andChlamydiatrachomatis.