聚合酶链反应(PCR)在结核分支杆菌检测中的应用日益广泛,结核菌模板的制备对于基因扩增至关重要。由于结核菌细胞壁脂类含量达 41%,其中大部分属蜡质,故该菌胞壁坚韧,一般的裂解剂不能将结核菌破坏,裂解效果不理想,传统的 DNA提取方法操作繁杂,不适于临床应用。所以探索快速、 Polymerase chain reaction (PCR) in the detection of Mycobacterium tuberculosis is increasingly widespread, preparation of TB template is essential for gene amplification. Due to the lipid content of the cell wall of TB is up to 41%, most of them are waxy, the wall of the cell is tough. The common lysing agent can not destroy the TB, the dissociation effect is not ideal. The traditional DNA extraction method is complicated and not suitable for Clinical application. So to explore fast,