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目的：探讨槲皮素（Ｑｕｅ）对血管平滑肌细胞胞内游离钙浓度（［Ｃａ~（２＋）］ｉ）的影响。方法：采用新一代钙荧光探剂 Ｆｌｕｏ－３／ＡＭ检测 Ｑｕｅ对培养的兔主动脉血管平滑肌细胞（ＡＳＭＣ）［Ｃａ~（２＋）］ｉ在高Ｋ~＋、去甲肾上腺素（ＮＥ）、血管紧张素Ⅱ（ＡｎｇⅡ）刺激作用下的改变，并与Ｖｅｒａｐａｍｉｌ进行对照研究。结果：Ｑｕｅ（１０~（－６）、１０~（－５）、１０~（－４）ｍｏｌ／１）呈剂量依赖性抑制高Ｋ~＋去极化引起的［Ｃａ~（２＋）］ｉ升高，与Ｖｅｒａｐａｍｉｌ作用相似，但弱于Ｖｅ－ｒａｐａｍｉｌ；Ｑｕｅ（１０~（－６）～１０~（－４）ｍｏｌ／Ｌ）对ＮＥ，ＡｎｇⅡ通过受体介导引起的［Ｃａ~（２＋）］ｉ增高也具有明显的抑制作用。但Ｑｕｅ对静息状态的ＡＳＭＣ［Ｃａ~（２＋）］ｉ无明显影响。结论：Ｑｕｅ通过对血管平滑肌细胞电压依赖性钙通道和受体操纵性钙通道双重抑制作用，降低细胞内游离钙水平，这可能是其舒血管降压机制之一。
Objective: To investigate the effect of quercetin on intracellular free calcium concentration ([Ca ~ (2 +)] i) in vascular smooth muscle cells. Methods: Fluorescence probe Fluo-3 / AM was used to detect the effect of Que on the expression of [Ca ~ (2 +)] i in rabbit aortic vascular smooth muscle cells (ASMCs) Angiotensin Ⅱ (AngⅡ) stimulated changes, and with Verapamil control study. RESULTS: Que (10 -6, 10 -5, 10 -4 mol / 1) inhibited the [Ca 2+] i induced by high K + depolarization in a dose-dependent manner (10 ~ (-6) ~ 10 ~ (-4) mol / L) on the expression of [Ca ~ (2 +)] )] I increased also has a significant inhibitory effect. But Que had no significant effect on resting ASMC [Ca ~ (2 +)] i. CONCLUSION: Que inhibits the intracellular free calcium level by double inhibition of voltage-dependent calcium channel and receptor-operated calcium channel in vascular smooth muscle cells, which may be one of the mechanisms of vasodilatation.