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RAPD分析技术即random amplified polymorphic DNA,是以PCR基本程序和DGGE(denaturing gradient gelelctrophore- sis,变性梯度凝胶电泳)为基础开发出的一种检测核苷酸变性的方法。它是1990年美国杜邦公司科学家Williams和加利福尼亚生物研究所Welsh领导的两个小组几乎同时发展起来的一项技术。有人认为它与常规PCR相比,主要有以下特点:①无需专门设计RAPD扩增反应的引物,也不须预
The RAPD analysis technique is random amplified polymorphic DNA, which is a method for detecting nucleotide denaturation based on the basic PCR program and DGGE (denaturing gradient gradient gel electrophoresis). It was a technique that was developed almost simultaneously by two groups led by Williams of the DuPont company in the United States and Welsh of the California Institute of Biology in 1990. Some people think that it has the following characteristics compared with conventional PCR: 1 There is no need to specifically design primers for RAPD amplification reaction, nor to pre