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利用田间试验对67份野生大豆材料进行草甘膦抗性筛选,结果在草甘膦有效剂量为1.23 kg.hm-2条件下,不同野生大豆对草甘膦抗性存在明显差异,其中ZYD0685和ZYD2405的存活率最高,分别为87%和83%;7份材料的存活率为2.7%～38%;58份材料死亡。以抗性材料ZYD0685和敏感材料ZYD0790进行生理指标研究,结果在未经草甘膦处理时,二者的叶绿素含量、莽草酸含量及谷胱甘肽-S-转移酶(GSTs)活性基本相同。在草甘膦不同剂量处理下,抗性材料ZYD0685叶绿素和莽草酸含量没有明显变化,GSTs活性明显增加;而敏感材料ZYD0790莽草酸含量明显升高,叶绿素含量明显降低,GSTs活性变化幅度较小。因此,植物体内莽草酸积累量较少是抗草甘膦的主要生理反应。
Sixty-seven wild soybean cultivars were selected for glyphosate resistance test. The results showed that the glyphosate resistance of wild soybean differed significantly with the effective dose of glyphosate being 1.23 kg · hm-2. Among them, ZYD0685 and The highest survival rate of ZYD2405 was 87% and 83% respectively; the survival rate of 7 materials was 2.7% ~ 38%; 58 materials were dead. Physiological indexes of resistant material ZYD0685 and sensitive material ZYD0790 were studied. As a result, chlorophyll content, shikimic acid content and glutathione S-transferase (GSTs) activity of the two plants were basically the same when they were not treated with glyphosate. Under the different doses of glyphosate, the content of chlorophyll and shikimic acid of resistant material ZYD0685 did not change obviously and the activity of GSTs increased obviously. However, the content of shikimic acid of ZYD0790 increased obviously, the content of chlorophyll decreased significantly and the activity of GSTs changed less. Therefore, less accumulation of shikimic acid in plants is the major physiological response to glyphosate.