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目的:考察梓醇对血管性痴呆(VD)大鼠学习记忆的影响及作用机制。方法:采用双侧颈总动脉永久性结扎制备血管性痴呆模型,造模后第30d将大鼠分成模型组、梓醇(60、30、15mg/kg)组,尼麦角林片(7mg/kg)组和假手术组,连续给药30d。Morris水迷宫检测学习记忆,苏木精-伊红(HE)染色观察大鼠海马组织形态,测定海马乙酰胆碱酯酶(Ach E)、乙酰胆碱转移酶(Ch AT)、一氧化氮(NO)含量和一氧化氮合酶(NOS)的活性。结果:与模型组比较,梓醇(60、15mg/kg)组大鼠站台穿越次数明显增加,梓醇可减轻VD大鼠海马锥体细胞损伤,梓醇(60、30mg/kg)组海马Ach E、NO含量降低,梓醇60mg/kg组海马NOS活性降低。结论:梓醇能够改善VD大鼠的学习记忆能力,可能与改善胆碱能系统及降低NO含量、抑制NOS活性有关。
Objective: To investigate the effect and mechanism of Catalpol on learning and memory of vascular dementia (VD) rats. Methods: The model of vascular dementia was established by permanent ligation of bilateral common carotid arteries. At the 30th day after model establishment, the rats were divided into model group, catalpol group (60,30,15mg / kg), nicergoline group (7mg / kg ) Group and sham operation group, continuous administration 30d. Morris water maze was used to detect learning and memory. The hippocampus was observed by hematoxylin-eosin (HE) staining. The content of hippocampal acetylcholinesterase (AchE), acetylcholinesterase (ChAT) and nitric oxide (NO) Nitric oxide synthase (NOS) activity. Results: Compared with the model group, the number of platform crossing of catalpol group (60 and 15 mg / kg) significantly increased. Catalpol can reduce the damage of hippocampal pyramidal cells in VD rats. In cats of 60 and 30 mg / kg, E, NO content decreased, Catalpol 60mg / kg group decreased hippocampal NOS activity. Conclusion Catalpol can improve the learning and memory ability of VD rats, which may be related to the improvement of cholinergic system, the reduction of NO content and the inhibition of NOS activity.