在进行电镜样本的超薄切片前，为了克服超薄切片的盲目性和电镜视野的局限性对目的视野观察的影响，电镜工作者普遍采用半薄切片技术制作0．5－Zmp的半薄切片，对其进行染色后在光学显微镜下观察和选择超薄切片的部位。但是半薄切片是由树脂包埋过的，直接对其染色往往有一定的 In order to overcome the blindness of the ultrathin sections and the limitation of the electron field of view before the ultrathin sectioning of the electron microscope samples, the semi-thin section technique is generally used by the electron microscope workers to make the semi-thin section of 0.5-Zmp , Stained and observed under an optical microscope and selected ultra-thin section of the site. However, semi-thin slices are embedded by the resin, the direct staining of them often have a certain