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[Objective] To supplement the quality standard of HERBA PLANTAGINIS.[Method]TLC was adopted to identify HERBA PLANTAGINIS qualitatively,ethylacetate-petroleum ether(30-60 ℃)-formic acid(V/V,10:15:1) as developer and1% aluminium chloride-alcohol as color development reagent;the content of luteolin was determined by HPLC,and the HPLC conditions were as follows:Alltech Apollo C18(4.6 mm×250.0 mm,5 μm)column as chromatographic column,mobile phase of methanol-0.2% phosphoric acid(V/V,55:45),flow rate of 1.0 ml/min,detective wavelength of 349 nm,column temperature of 35 ℃.[Results]The test sample had the same fluorescence spots with luteolin standard and drug reference substance.Luteolin and peak area were in good linear relationship in the range of 1.05-21.00 μg/ml(r=0.999 8),and the average recovery rate of luteolin were 99.60%(RSD=1.70%).[Conclusion] The method established in this study is reliable,accurate and specific,which can be used for the quality control of HERBA PLANTAGINIS.
[Objective] To supplement the quality standard of HERBA PLANTAGINIS. [Method] TLC was adopted to identify HERBA PLANTAGINIS qualitatively, ethylacetate-petroleum ether (30-60 ° C) -formic acid (V / V, 10: 15: 1) as developer and 1% aluminum chloride-alcohol as color development reagent; the content of luteolin was determined by HPLC, and the HPLC conditions were as follows: Alltech Apollo C18 (4.6 mm × 250.0 mm, 5 μm) column as chromatographic column, mobile phase of methanol The flow rate of 1.0 ml / min, detective wavelength of 349 nm, column temperature of 35 ° C. [Results] The test sample had the same fluorescence spots with luteolin standard and -0.2% phosphoric acid (V / V, 55:45) drug reference substance. Luteolin and peak area were in good linear relationship in the range of 1.05-21.00 μg / ml (r = 0.999 8), and the average recovery rate of luteolin were 99.60% (RSD = 1.70% The method established in this study is reliable, accurate and specific, which can be used for the quality control of HERBA PLANTAGINIS .