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目的:探讨克罗恩病患者结肠粘膜组织中IL-23、IL-12的表达及其与疾病严重程度的相关性。方法:选取中南大学湘雅医院2005年4月-2011年3月经确诊的30例克罗恩病患者手术标本,对照选取30例结肠癌患者手术切除之正常结肠组织。实验设CD病变肠段组、CD正常肠段组及对照组3组。用免疫组织化学方法检测结肠内IL-12、IL-23的表达,分析IL-23、IL-12的表达与CD的疾病临床严重程度(CDAI)和内镜下肠黏膜损伤程度(CDEIS)的相关性。结果:IL-12的表达:CD病变肠段组、CD正常肠段组、对照组阳性率无统计学意义(P值>0.05)。IL-23的表达:CD病变肠段组阳性率高于CD正常肠段组,CD正常肠段组高于对照组,差异均有统计学意义(P值<0.05)。结论:IL-12在CD患者肠粘膜中不升高,而IL-23在CD表达明显升高,提示IL-23在CD粘膜免疫损伤中发挥一定作用,对CD的诊断有一定的参考价值。
Objective: To investigate the expression of IL-23 and IL-12 in colonic mucosa of patients with Crohn’s disease and its correlation with the severity of the disease. Methods: Thirty cases of Crohn’s disease diagnosed in Xiangya Hospital of Central South University from April 2005 to March 2011 were selected. Thirty normal colon tissues of patients with colon cancer underwent surgical resection. Experimental set CD lesion bowel group, CD normal bowel group and control group 3 groups. The expression of IL-12 and IL-23 in the colon was detected by immunohistochemical method. The expression of IL-23 and IL-12 was analyzed in relation to the clinical severity of CD disease and CDEIS Correlation. Results: The expression of IL-12 was not significantly different in the intestinal segment of CD lesion, normal bowel segment of CD, and control group (P> 0.05). The positive rate of IL-23 in CD group was higher than that in normal CD group and normal CD group (P <0.05). Conclusion: IL-12 is not increased in intestinal mucosa of CD patients, while the expression of IL-23 is significantly increased in CD, suggesting that IL-23 plays a role in the CD mucosal immune injury and may be of reference value for the diagnosis of CD.