论文部分内容阅读
目的:观察灵芝对糖尿病大鼠肾组织氧化应激的影响。方法:30只雄性SD大鼠随机分为3组:正常对照组(C组),糖尿病未治疗组(DM组),糖尿病灵芝(1.2g.kg-1)治疗组(GL组)。腹腔注射链脲佐菌素(STZ)诱导大鼠造成糖尿病模型,GL组用灵芝进行治疗性灌胃。8周后,所有大鼠一次性处死。检测肾组织丙二醛(MDA)的含量、肾组织超氧化物歧化酶(SOD)及过氧化氢酶(CAT)与谷胱甘肽过氧化物酶(GSH-Px)活性;同时进行光镜、透射电镜观察各组大鼠肾皮质病理改变。结果:DM组大鼠出现糖尿病肾病(DN)典型的病理改变。GL组肾组织MDA含量明显低于DM组,SOD、CAT、GSH-Px活性明显高于DM组(P<0.01),病理改变好转。结论:灵芝可能通过抑制肾组织氧化应激,起到对糖尿病大鼠肾脏的保护作用。
Objective: To observe the effect of Ganoderma lucidum on oxidative stress in kidney of diabetic rats. Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control group (C group), diabetic untreated group (DM group), and diabetic ganoderma lucidum (1.2g.kg-1) treatment group (GL group). Intraperitoneal injection of streptozotocin (STZ) induced a diabetic model in rats, and GL group was treated with Ganoderma for therapeutic gavage. After 8 weeks, all rats were sacrificed at one time. Detection of malondialdehyde (MDA) content in kidney tissue, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity in renal tissue; simultaneous light microscopy Transmission electron microscope was used to observe the pathological changes of renal cortex in rats. Results: Diabetic nephropathy (DN) typical pathological changes occurred in DM rats. The MDA content in renal tissue of GL group was significantly lower than that of DM group, and the activities of SOD, CAT and GSH-Px were significantly higher than those of DM group (P<0.01), and the pathological changes were improved. Conclusion: Ganoderma lucidum may protect kidney of diabetic rats by inhibiting oxidative stress in kidney tissue.