为探索生物固氮机理,国际上对各种来源固氮酶催化基质还原的抑制试验早有不少报道,然而不论是经典抑制剂还是几种成对基质之间的抑制类型,国外不同实验室得到的结果很不相同;作为我国固氮研究常用菌株的棕色固氮菌(NO.230),其固氮酶对基质还原的抑制作用和有关参数,国内至今尚未见报道;因此我们在前文基质还原的动力学基础上,选择几项典型的抑制试验进行系统测试,颇有必要。 In order to explore the mechanism of biological nitrogen fixation, there are many reports on the inhibition of nitrogenase-catalyzed substrate reduction by various sources in the world. However, whether it is a classical inhibitor or a type of inhibition between several pairs of substrates, The results are very different. As a nitrogen-fixing strain commonly used in our country, Azotobacter vinelandii (NO.230), its nitrogenase inhibitory effect on matrix reduction and related parameters have not been reported so far in our country. Therefore, we have previously described the kinetics of matrix reduction On the choice of several typical suppression test for system testing, it is quite necessary.