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目的 :建立高效液相色谱法测定肠必清肠溶颗粒剂中番泻苷A、B含量的方法。方法 :以C18 柱为分析柱 ,以50 %乙腈 (含0 25mmol/L十六烷基三甲基溴化铵和0 25mmol/L磷酸氢二钠 ,每200ml加入冰醋酸75μl)为流动相 ,流速为1ml/min ,检测波长为270nm。结果 :在0 25μg/ml～80 0μg/ml浓度范围内呈线性关系 (番泻苷A :r=0 9997 ;番泻苷B :r=0 9997) ,番泻苷A、B测定方法重现性较好 (RSD<2 % )的精密度均<2 % ,回收率均>98 %。结论 :本方法简单、准确、重现性好 ,可以用于肠必清肠溶颗粒剂的质量控制
Objective: To establish a method for the determination of sennoside A and B in Changbai Qingchang Granules by HPLC. Methods: The mobile phase consisted of C18 column, 50% acetonitrile (containing 0 25 mmol / L cetyltrimethylammonium bromide and 0 25 mmol / L disodium hydrogen phosphate, and 200 μl of glacial acetic acid per 200 ml) The flow rate was 1 ml / min and the detection wavelength was 270 nm. Results: There was a linear relationship between the concentrations of 0 25 μg / ml and 80 0 μg / ml (sennoside A: r = 0 9997; sennoside B: r = 0 9997) The better the sex (RSD <2%), the precision was <2% and the recovery rate was> 98%. Conclusion: The method is simple, accurate, reproducible, and can be used for quality control of Changbai Qingchang Granules