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以白菜小孢子为试材,以B5培养基为基本培养基,研究胚状体诱导期、幼苗生长期,添加不同浓度蔗糖、琼脂、2,4-D、6-BA、马铃薯和弱光培养时间对外植体产生玻璃化及褐变的影响。结果表明:在胚状体诱导期,糖浓度较低时和添加2,4-D均可增加玻璃化的发生率。培养3、5、7、9d的玻璃化率分别为52.00%、37.50%、23.91%和22.92%,褐变率分别为20.00%、14.58%、8.69%和8.33%,3、5、7d处理间存在极显著差异;较高浓度的糖、琼脂及添加马铃薯对抑制幼苗玻璃化的产生有利;6-BA可明显促进玻璃化的发生,且随浓度的增加,幼苗玻璃化程度明显加重。除弱光培养时间外,其它因素对褐变率的影响均较小。
Using cabbage microspore as test material and B5 medium as basic medium, embryogenesis, seedling growth and embryogenesis were studied. The effects of different concentrations of sucrose, agar, 2,4-D, 6-BA, Effect of time on vitrification and browning of explants. The results showed that the incidence of vitrification could be increased in the induction of embryoid body, when the concentration of sugar was low and when 2,4-D was added. The glass transition rates were 52.00%, 37.50%, 23.91% and 22.92% at 3, 5, 7 and 9 days respectively. The browning rates were 20.00%, 14.58%, 8.69% and 8.33% 6-BA could significantly promote the vitrification. With the increase of concentration, the degree of vitrification of seedling obviously increased. In addition to the weak light culture time, other factors on the browning rate are small.