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目的研究经胃灌入高氧液(HO)对油酸型急性肺损伤(ALI)家兔的治疗作用。方法以复方氯化钠为基液制备HO。将18只家兔随机均分为油酸致伤组(A组)、HO治疗组(B组)和正常对照组(C组)。A、B组均经耳缘静脉注入油酸0.06ml/kg建立ALI模型,C组注入等量生理盐水。B组于注射油酸30min时经胃灌入HO20ml/kg,A、C组灌入等量复方氯化钠。各组分别在ALI模型制备前(0min)、注射油酸后30、60、120min经右颈外动脉抽血进行血气分析,并测定肺水含量、肺体比值及病理检查。结果与C组相比,A组PaO2、PaCO2明显降低(P<0.01),肺水含量、肺体比值及肺损伤评分明显增高(P<0.01),镜下见肺灶性出血,间质、肺泡水肿,大量炎性细胞浸润。B组经HO治疗后迅速提升PaO2、PaCO2(P<0.05),减轻肺水肿(P<0.01)。结论经胃灌入HO对油酸型ALI家兔有治疗作用。
Objective To study the therapeutic effect of intragastric infusion of hyperoxia liquid (HO) on rabbits with oleic acid-induced acute lung injury (ALI). Methods Compound sodium chloride as a base solution to prepare HO. 18 rabbits were randomly divided into oleic acid injury group (A group), HO treatment group (B group) and normal control group (C group). The ALI model was established by injection of oleic acid 0.06ml / kg into the A and B groups, and the same amount of saline was injected into the C group. Group B was infused with 20 mL / kg of HO via stomach at the time of oleic acid injection for 30 min. Groups A and C were infused with the same amount of compound sodium chloride. Blood samples were taken from the right external carotid artery before injection of oleic acid (30 min, 60 min and 120 min after injection of oleic acid) before ALI model preparation (0 min), and lung water content, pulmonary body mass ratio and pathological examination were determined. Results PaO2 and PaCO2 in group A were significantly lower than those in group C (P <0.01), lung water content, lung ratio and lung injury score were significantly increased (P <0.01) Alveolar edema, a large number of inflammatory cell infiltration. In group B, PaO2 and PaCO2 were rapidly increased (P <0.05) and pulmonary edema was relieved after treatment with HO (P <0.01). Conclusion Oral gastric infusion of HO on oleic ALI rabbits have a therapeutic effect.