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目的探讨青藤碱免疫抑制作用,为其在器官移植免疫抑制治疗上的应用提供实验依据。方法建立48个大鼠原位肾移植急性排斥反应模型(Wistar-SD)分为四组分别于腹腔注射生理盐水(1ml/(kg.d))、青藤碱(30mg/(kg.d))、环孢菌素A(2.5mg/(kg.d))、青藤碱(30mg/(kg.d))+环孢菌素A(2.5mg/(kg.d)),每组12例,随机抽取6例观察受者存活时间,另6例于术后第六天处死,取腔静脉血测尿素氮及肌酐,取移植肾组织标本病检。分析青藤碱协同环孢菌素A对大鼠肾移植受者存活时间、肾功能指标BUN、Cr以及对移植肾组织病理变化的影响。结果青藤碱[30mg/(kg·d)]可延长受体鼠存活时间至(9.67±0.52)d,与环孢菌素A[2.5mg/(kg·d)]联用后可明显延长受体鼠存活时间至18天以上(25.00±4.65)d;青藤碱对移植肾的功能表现出保护作用并改善移植肾组织的病理变化。结论青藤碱对大鼠肾移植的急性排斥反应起到一定的抑制作用,并与亚治疗剂量的环孢菌素A产生协同作用,青藤碱是一种从自然药物中提取的具有开发前景的免疫抑制药物。
Objective To investigate the immunosuppressive effects of sinomenine and provide experimental basis for its application in immunosuppressive therapy of organ transplantation. Methods Forty-eight rat models of acute orthotopic renal transplantation rejection (Wistar-SD) were divided into four groups: intraperitoneal injection of normal saline (1 ml/(kg.d)) and sinomenine (30 mg/(kg.d). ), Cyclosporin A (2.5 mg/(kg.d)), Sinomenine (30 mg/(kg.d)) + Cyclosporin A (2.5 mg/(kg.d)), 12 per group For example, 6 patients were randomly selected to observe the survival time of the recipient, and the other 6 patients were sacrificed on the sixth day after operation. The venous blood was measured for urea nitrogen and creatinine, and the renal tissue specimens were taken for examination. To analyze the effects of sinomenine and cyclosporin A on the survival time, renal function index BUN, Cr, and pathological changes of renal allograft recipients. Results Sinomenine [30 mg/(kg·d)] prolonged the survival time of the recipient mice to (9.67±0.52) days, which was significantly prolonged when combined with cyclosporin A [2.5 mg/(kg·d)]. The survival time of the recipient mice was more than 18 days (25.00±4.65) days. The sinomenine showed protective effects on the transplanted kidney and improved the pathological changes of the transplanted kidney tissue. Conclusion Sinomenine has a certain inhibitory effect on acute rejection of renal transplantation in rats, and synergizes with sub-therapeutic dose of cyclosporin A. Sinomenine is a promising drug extracted from natural drugs. Immunosuppressive drugs.