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用连续传代的人T细胞系、人外周血单个核细胞或人单核/巨噬细胞作靶细胞已建立了几种不同的评价抗病毒物质抗人类免疫缺陷症病毒(HIV)的方法,多数方法是以病毒对靶细胞的作用(如合胞体形成、细胞死亡或病毒颗粒的释放)为终点测定指标。然而,对增殖病毒的定量检测,容斑试验可能是最理想的方法。作者用MT-4细胞容斑试验(PA)及CD4~+Hela细胞灶免疫试验(FIA)对几种化合物的抗HIV-1活性进行了评价和比较。PA是将MT-4细胞粘附到多聚L-赖氨酸包被的板上,计数病毒所致的容斑。作者
Several different methods of evaluating anti-viral substances against human immunodeficiency virus (HIV) have been established with serial passage of human T-cell lines, human peripheral blood mononuclear cells or human monocytes / macrophages as target cells, and most Method is based on the role of the virus on the target cells (such as syncytia formation, cell death or release of viral particles) as the end point determination of indicators. However, the quantitative detection of proliferating virus, spot test may be the most ideal method. The authors evaluated and compared the anti-HIV-1 activity of several compounds using the MT-4 Cell Plaque Assay (PA) and the CD4 ~ + Hela Focal Cell Immune Assay (FIA). PA binds MT-4 cells to poly-L-lysine coated plates and counts virus-induced plaque. Author