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本文研究了单一因素、复方因素以及综合因素对芸薹属芥菜型(Brassica juncea)自生种质R001深休眠种子的破眠效果。结果表明:(1)单一因素的破眠方法,无论是物理因素(针刺、变温、风干或干热处理),或者是化学因素(C_2H_5OH、H_2O_2、NaNO_3或GA_3),对于解除R001种子的深休眠,效果不甚显著,发芽率在24.09％以下。(2)复方的破眠方法,包括风干结合GA_2、风干结合变温以及变温结合GA,对R001种子的萌发具有明显的促进作用,发芽率可以达到45.43％以上,其中又以GA_3给合变温处理效果较佳。(3)用干(25—30天)、GA_3(200ppm)、变温(4—30℃)三者相结合的综合破眠方法,能使全部R001深休眠种子的萌发,发芽率为100％,破眠程度(DBE)值达到1.00。
In this paper, the effect of single factor, compound factor and comprehensive factor on the hypnotic effect of deep dormancy seed R001 of Brassica juncea autotrophic germplasm were studied. The results showed that: (1) The single-factor sleep-deprivation method, no matter physical factors (acupuncture, temperature change, air-dry or dry heat treatment) or chemical factors (C_2H_5OH, H_2O_2, NaNO_3 or GA_3) , The effect is not significant, the germination rate of 24.09% or less. (2) The method of hypnosis of compound, including air-drying combined with GA 2, air-drying combined with variable temperature and combination of variable temperature and GA, could obviously promote the germination of R001 seeds, the germination rate could reach more than 45.43% Better. (3) The integrated dormancy method combined with dry (25-30 days), GA 3 (200ppm), variable temperature (4-30 ℃) can make the germination and germination rate of 100% Depth of Sleep (DBE) value of 1.00.