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目的克隆回本血吸虫中国大陆株２１．７ｋＤａ分子的编码基因。方法合成１对特异性引物，通过聚合酶链反应技术从日本血吸虫中国大陆株ｃＤＮＡ中对目的基因进行扩增和克隆，对扩增产物进行核苷酸序列分析。结果用聚合酶链反应，从日本血吸虫中国大陆株ｃＤＮＡ中扩增出１条单一的ＤＮＡ条带，大小约为 ５００～６００ ｂｐ。核苷酸序列分析表明日本血吸虫中国大陆株 ２１． ７ ｋＤａ分子的开放阅读框全长为５５８ ｂｐ，与日本血吸虫菲律宾株２１． ７ ｋＤａ分子基因完全同源。结论日本血吸虫中国大陆株 ２１．７ ｋＤａ膜蛋白基因克隆成功为进一步研究该分子用作疫苗的潜能奠定基础。
Objective To clone the gene encoding 21.7 kDa molecule of Schistosoma japonicum in Chinese mainland. Methods A pair of specific primers was synthesized and the target gene was amplified and cloned from cDNA of Schistosoma japonicum strain by polymerase chain reaction (PCR). The nucleotide sequence of the amplified product was analyzed. Results A single DNA band was amplified from cDNA of Schistosoma japonicum by polymerase chain reaction (PCR). The size of DNA was about 500-600 bp. Nucleotide sequence analysis showed that Schistosoma japonicum Chinese mainland strain 21. The full-length open reading frame of the 7 kDa molecule was 558 bp in length, and the S. japonicum Philippines strain 21. 7 kDa molecular gene completely homologous. Conclusion The successful cloning of the 21.7 kDa membrane protein gene of Schistosoma japonicum in Chinese mainland lays the foundation for further study on the potential of the molecule as a vaccine.