氢化酶是可逆分裂重组氢分子的一类酶 .由于在燃料细胞和新能源方面具有诱人的应用前景 ,成为当今自然界向能源、化学学科挑战课题之一 .目前发现的 4种氢化酶中 ,镍铁氢化酶的存在最为普遍 ,研究也最为深入 .但在活性中心结构组成和催化机理方面一直存在着不同观点和争论 ,引起许多国家学者的极大关注 . Hydrogenase is a type of enzyme that reversibly cleaves recombinant hydrogen molecules and has become one of the most challenging topics in the field of energy and chemistry due to its attractive application prospect in fuel cells and new energy sources.Of the four hydrogenase enzymes found so far, The existence of ferronickel is the most common and the study is the most in-depth.However, there have been different opinions and controversies in the structure and catalytic mechanism of active center, which aroused great concern of many national scholars.