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目的 探讨高原高寒环境下平战时肢体火器伤后血浆及伤道组织中总抗氧化能力(T AOC)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量变化的规律。方法 将2 4头实验猪随机分为高原战时致伤组(GZ)、高原平时致伤组(GP)和平原平时致伤组(PP) ,每组8头,在不同环境下致伤;分别于伤前、伤后不同时相点采集中静脉血和伤道肌肉组织定量测定T AOC、SOD、MDA。结果 ①平原平时组血浆和伤道组织内T AOC伤后均持续下降,在伤后1～2d降至最低点,此后逐渐上升;SOD、MDA均在伤后3 0min～2h即有升高,至6h时达最高点,此后逐渐下降,组织中3d恢复到较低水平,血浆中5d恢复至正常水平。②高原平时组血浆和伤道组织内T AOC、SOD、MDA变化规律与平原平时组相似,但变化的幅度大、持续的时间长。③高原战时组与高原平时组相比,血浆和伤道组织内T AOC在伤前即有显著降低,SOD、MDA显著增高,伤后各指标升高或降低的程度、持续时间均显著高于高原平时组,峰值出现的时间也有所提前。结论 高原高寒战时环境猪肢体枪弹伤后全身和局部伤道组织的损伤程度重于高原平时组,而高原平时组的损伤又重于平原平时组。
Objective To investigate the changes of total antioxidant capacity (T AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) in plasma and wound tissues after firearm wounds in Ping-wart period in the high altitude and alpine region. Methods Twenty - four experimental pigs were randomly divided into three groups: the wounded group (GZ), the normal group (GP) and the plain group (8), which were injured in different environments. T AOC, SOD and MDA were measured quantitatively in the venous blood and injured muscle tissues at different time points before and after injury respectively. Results ① The levels of plasma and wound tissue in the plain group were decreased continuously after T AOC injury and decreased to the lowest point at 1 ~ 2 d after injury, then gradually increased. SOD and MDA increased after 30 min to 2 h after injury, Reached the highest point at 6h, then gradually decreased, 3d in the tissue returned to a lower level, 5d plasma returned to normal levels. ② The changes of T AOC, SOD and MDA in the plasma and wound tissue in the normal plateau group were similar to those in the plain group, but the changes ranged in length and continued for a long time. (3) T AOC in plasma and wound tissue were significantly lower than those in normal group at high altitude in wartime group, while the levels of SOD and MDA were significantly increased, the extent of increase or decrease of each index was significantly higher In the usual plateau group, the peak appeared earlier. Conclusion The damage of whole body and local wound tissue after pig-limb gunshot wounds in the high-altitude wartime environment was heavier than that in the normal plateau group, while the injury in the normal plateau group was heavier than that in plain group.