论文部分内容阅读
目的 研究氧化苦参碱对小鼠免疫性肝纤维化的作用机制。方法 将BABL/c小鼠随机分成正常对照组、阳性对照组、氧化苦参碱小剂量、大剂量治疗组 ,阳性对照组和氧化苦参碱治疗组每周静脉注射 1次刀豆蛋白A ,共 2 0周。氧化苦参碱治疗组分别腹腔注射氧化苦参碱 3 0mg/kg、60mg/kg。共 2 0周。分别于注射刀豆蛋白A后 5、1 2和 2 0周取血低温保存 ;并每次处死一批小鼠取肝脏组织液氮低温保存 ,常规HE染色、VanGieson胶原纤维染色 ,冰冻切片CD4 + 、CD8+ T淋巴细胞染色。图像分析系统定量分析肝内纤维组织含量。结果 氧化苦参碱治疗组血清ALT含量及肝组织内炎症活动度、纤维含量均低于阳性对照组 ,而且呈剂量依赖性。结论 氧化苦参碱有减轻小鼠肝脏炎症活动度及抗小鼠免疫性肝纤维化的作用。
Objective To study the mechanism of oxymatrine on immune hepatic fibrosis in mice. Methods BABL / c mice were randomly divided into normal control group, positive control group, oxymatrine low dose, high dose treatment group, positive control group and oxymatrine treatment group intravenous injection of concanavalin A, A total of 20 weeks. Oxymatrine treatment group were intraperitoneal injection of oxymatrine 30mg / kg, 60mg / kg. A total of 20 weeks. Blood samples were stored at low temperature for 5, 12 and 20 weeks after the injection of concanavalin A, respectively. Each group of mice was sacrificed and the liver tissue was stored in liquid nitrogen for storage at low temperature. The specimens were stained with HE, VanGieson collagen fibers, CD8 + T lymphocyte staining. Image analysis system Quantitative analysis of intrahepatic fiber content. Results In the oxymatrine treatment group, serum ALT level, inflammatory activity and fiber content in the liver tissue were lower than those in the positive control group and in a dose-dependent manner. Conclusion Oxymatrine can reduce the activity of hepatic inflammation and anti-mouse immunological liver fibrosis in mice.