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目的:研究桑牛(SN)强生胶囊对γ射线辐射的防护作用,为研制防治辐射损伤的药食两用制品提供理论依据。方法:雄性昆明健康小鼠随机分为正常对照组、辐射模型组与SN强生胶囊制品大中小3个剂量组,共5组。灌胃21d,于给药18d后,分别以4.0和7.0Gy60Coγ射线全身照射小鼠造成辐射损伤后,分别测定各组小鼠脾脏及胸腺重量指数、外周血WBC数量及SOD活力与MDA含量。结果:照射后第3天和第14天,SN强生胶囊大剂量组小鼠白细胞总数与辐射模型组比较差异均有统计学意义,P<0.05。SN强生胶囊大、中剂量组与辐射模型组比较,SN强生胶囊能显著提高辐射小鼠的胸腺、脾脏免疫脏器重量指数,P<0.05。辐射后使小鼠胸腺、脾脏的MDA水平明显升高,SOD活力明显下降(P<0.05),补充SN强生胶囊后,SN强生胶囊大、中剂量组脾脏和胸腺MDA水平分别降低,与辐射模型组比较差异有统计学意义,P<0.05;使脾脏和胸腺SOD活性较辐射模型组分别提高,变化均具有统计学意义,P<0.05。结论:SN强生胶囊在一定程度上可减缓由辐射引起的小鼠外周血白细胞细胞数下降,可明显提高电离辐射机体的抗氧化能力,进而提高机体抗辐射能力。
Objective: To study the protective effect of mulberry (SN) Johnson Capsule on γ-ray radiation and to provide a theoretical basis for the development of dual-purpose products for preventing and treating radiation injury. Methods: Male Kunming healthy mice were randomly divided into normal control group, radiation model group and SN Johnson Capsule products three groups, a total of 5 groups. After intragastric administration for 21 days, the mice were irradiated with 4.0 and 7.0Gy of 60Coγ ray respectively after radiation for 18 days. After radiation injury, the spleen and thymus weight index, peripheral blood WBC count, SOD activity and MDA content were determined. Results: On the 3rd and 14th day after irradiation, the total number of leukocytes in the mice treated with high doses of SN Johnson Capsule was significantly lower than that in the radiation model group (P <0.05). Compared with radiation model group, SN Johnson capsule could significantly increase the weight index of immune organ in thymus and spleen of irradiated mice, P <0.05. After irradiation, the MDA level in thymus and spleen of mice increased significantly and the activity of SOD decreased significantly (P <0.05). After SN Johnson capsules were added, the levels of MDA in spleen and thymus of SNJ group were decreased respectively. Compared with radiation model The difference was statistically significant (P <0.05). The activity of SOD in spleen and thymus was higher than that in radiation group (P <0.05). CONCLUSIONS: SN Johnson Capsule can slow down the decrease of peripheral blood leukocyte count caused by radiation in a certain extent, which can obviously improve the anti-oxidation ability of ionizing radiation and improve the anti-radiation ability of the body.