论文部分内容阅读
目的 检测宫颈鳞癌中KAI1在mRNA水平的表达,探讨其与宫颈癌侵袭转移的关系。方法 采用逆转录-多聚酶链反应(RT-PCR)技术检测新鲜组织KAI1 mRNA的表达,宫颈鳞癌组52例,正常对照组21例。结果 (1) KAI1 mRNA 在正常宫颈上皮及宫颈癌组织中阳性率分别为100%,61.53%,差异具有显著意义(P<0.05)。(2) 在高、中、低分化宫颈癌组织中KAI1 mRNA阳性率分别为76.47%、68.18%和30.70%,三组间差异有极显著意义(P<0.01)。(3) KAI1 mRNA在≤ⅡA、ⅡB、Ⅲ/Ⅳ期宫颈癌组织中表达阳性率分别为68.00%、55.56%和55.56%,阳性率在各期间差异无显著意义(P>0.05)。(4) KAI1 在无淋巴转移组的阳性率(27/34,79.41%)明显高于有淋巴转移组(5/18,27.78%),差异有显著意义(P<0.05)。结论 KAI1 mRNA在宫颈鳞癌组织中表达减少,其表达与细胞分化及转移有关,提示KAI1可能参与了宫颈癌的发生发展,可能在一定程度上具有预测宫颈癌侵袭转移的潜能。
Objective To detect the expression of KAI1 in cervical squamous cell carcinoma and its relationship with the invasion and metastasis of cervical cancer. Methods The expression of KAI1 mRNA in fresh tissues was detected by reverse transcription polymerase chain reaction (RT-PCR). Among 52 cases of cervical squamous cell carcinoma and 21 cases of normal control group, Results (1) The positive rates of KAI1 mRNA in normal cervical epithelium and cervical cancer tissues were 100% and 61.53%, respectively, with significant difference (P <0.05). (2) The positive rates of KAI1 mRNA in high, moderate and poorly differentiated cervical cancer tissues were 76.47%, 68.18% and 30.70%, respectively. There was significant difference among the three groups (P <0.01). (3) The positive rates of KAI1 mRNA expression in cervical cancer tissues of ≤ⅡA, ⅡB and Ⅲ / Ⅳ were 68.00%, 55.56% and 55.56%, respectively. There was no significant difference in the positive rates of KAI1 between different periods (P> 0.05). (4) The positive rate of KAI1 in non-lymphatic metastasis group (27/34, 79.41%) was significantly higher than that in lymphatic metastasis group (5/18, 27.78%), the difference was significant (P <0.05). Conclusions The expression of KAI1 mRNA is decreased in cervical squamous cell carcinoma and its expression is related to the differentiation and metastasis of cervical squamous cell carcinoma. It suggests that KAI1 may be involved in the occurrence and development of cervical cancer and may have the potential to predict the invasion and metastasis of cervical carcinoma to a certain extent.