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目的 探讨60 Coγ射线诱导人HPRT基因突变的分子图谱和发生机理 ,以及与辐射抗肿瘤的关系。方法 采用单细胞克隆培养、双向筛选计数、多重PCR扩增和电泳分析等方法。结果①随着辐射剂量的增加 ,细胞接种存活率下降 ,突变频率升高。②自发突变中 92 3%是点突变 ,而γ射线诱发突变主要由缺失与点突变两部分组成 (两者分别为 6 1 7%和 38 3 % )。③缺失突变可以发生于HPRT基因的每个外显子 ,但诱发突变中绝大多数是多个外显子的连锁缺失 (97 9% )。结论γ射线诱发HPRT基因突变图谱与自发突变不一样 ,其易发生较大遗传结构改变的特点与作用机理有关
Objective To investigate the molecular map and mechanism of human HPRT gene mutation induced by 60 Co γ-rays, and its relationship with radiation anti-tumor. Methods Single cell clonal culture, two-way screening, multiplex PCR amplification and electrophoresis analysis were used. Results 1 With the increase of radiation dose, the survival rate of cell inoculation decreased and the frequency of mutation increased. 2 Among the spontaneous mutations, 92% were point mutations, while γ-ray induced mutations were mainly composed of deletions and point mutations (both were 617 and 383%). 3 deletion mutations can occur in every exon of the HPRT gene, but most of the induced mutations are the linkage deletions of multiple exons (97 9%). Conclusions The gamma ray-induced HPRT gene mutation map is not the same as the spontaneous mutation. The susceptibility to changes in the genetic structure is related to the mechanism of action.