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目的:观察肿瘤坏死因子（TNF）对培养的牛脑微血管内皮细胞释放内皮素-1（ET－1）的刺激作用及粉防己碱（Tet）对ET－1释放的抑制作用。方法：放免测定法。结果：TNF在（l～100μg·L(-1)）之间能剂量依赖性的促进牛脑微血管内皮细胞释放ET-1，Tet（10(－6)～10(－4)mol·L(－1)）能拮抗TNF诱导的ET－1释放并呈量效关系。
Objective: To observe the effects of tumor necrosis factor (TNF) on the release of endothelin-1 (ET-1) from cultured bovine microvascular endothelial cells and the inhibitory effect of tetrandrine on the release of ET-1. Methods: RIA method. Results: TNF (1 ~ 100μg · L -1) promoted the release of ET-1 from bovine brain microvascular endothelial cells in a dose-dependent manner. The levels of ET (10 -6) -10 (-4) mol·L -1)) antagonized TNF-induced ET-1 release and showed a dose-response relationship.