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在NIH3T3细胞中表达了HBV PreS与碱性磷酸酶融合蛋白。所表达融合蛋白具有HBV PreS_1蛋白的抗原性和碱性磷酸酶活性,其产量可达33mg/L。该融合蛋白可用来筛选肝细胞cDNA表达文库克隆HBV肝细胞受体。
HBV PreS and alkaline phosphatase fusion proteins were expressed in NIH3T3 cells. The expressed fusion protein has the antigenicity and alkaline phosphatase activity of HBV PreS_1 protein and the yield can reach 33 mg / L. The fusion protein can be used to screen hepatocyte cDNA expression clones for HBV hepatocyte receptors.