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应用双重系统能够控制细胞和动物体内转基因表达的时空专一性，有利于分析基因功能和表达调节。病毒、细菌和酵母的调节系统可能用以控制哺乳动物细胞的基因表达．由此建立的双重系统经过外源激活物结合专一位置，刺激增强子／启动子，抑或经过外源重组酶识别和切除含重组位置的介入顺序，由此激活原来静止靶基因，引导组织细胞和发育时期专一表达．这种方法能克服导入毒性基因致使小鼠患病早死等困难。
The dual system can control the spatiotemporal specificity of transgene expression in cells and animals, which helps to analyze gene function and expression regulation. Regulatory systems of viruses, bacteria and yeasts may be used to control the gene expression in mammalian cells. The dual system thus established binds a specific site via an exogenous activator, stimulates an enhancer / promoter, or recognizes and excises an interventional sequence containing a recombination site via exogenous recombinase, thereby activating the original quiescent target gene and directing the tissue cells And the specific expression of developmental period. This method can overcome the importation of virulence genes cause mice premature death and other difficulties.