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目的:观察人参有效单体对活化的肾系膜细胞株的影响。方法:采用体外培养肾系膜细胞株的方法,将其分为空白对照组、TGF-β12ng/ml组、TGF-β12ng/ml+中药10-5g/ml组和TGF-β12ng/ml+中药10-6g/ml组。采用MTT法观察24h中药单体对系膜细胞株的作用以及ELISA法对TGF-β的分泌情况。结果:与空白对照组相比,加入TGF-β1刺激因子可以促进系膜细胞株的活化(P<0.05),以及TGF-β的分泌(P<0.01),中药单体可以抑制活化的细胞株增殖和TGF-β的分泌(P<0.05或P<0.01)。结论:人参中药单体可以抑制活化的肾系膜细胞株的增殖,有效防治肾纤维化,其可能的作用机制与其抑制TGF-β的分泌有关。
Objective: To observe the effect of ginseng effective monomer on activated mesangial cell line. Methods: The mesangial cell line was cultured in vitro and divided into blank control group, TGF-β12ng / ml group, TGF-β12ng / ml + TCM 10-5g / ml group and TGF- β12ng / ml + TCM 10-6g / ml group. MTT method was used to observe the effect of Chinese herbal medicine on mesangial cell lines and the secretion of TGF-β by ELISA. Results: Compared with the blank control group, the addition of TGF-β1 stimulating factor could promote the activation of mesangial cell line (P <0.05) and the secretion of TGF-β (P <0.01) Proliferation and secretion of TGF-β (P <0.05 or P <0.01). Conclusion: Ginseng Chinese traditional medicine can inhibit the proliferation of activated mesangial cell line and prevent and treat renal fibrosis effectively. Its possible mechanism is related to inhibiting the secretion of TGF-β.