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本实验运用屈肌反射-HRP逆行追踪结合实验,对电针促进大鼠坐骨神经干中躯体运动及感觉纤维成分的再生进行了观察。结果显示:电针组足底施予45±5V屈肌反射阈刺激,可引起短潜伏期的A类及长潜伏期的C类纤维传入反应;对照组足底经65±5V屈肌反射阈刺激后,仅见C类纤维传入所引起的屈肌反射。电针组右侧脊神经节内主要为大、小两种圆形细胞被标记,而对照组右侧脊神经节内标记细胞却均为淡染的小圆形细胞。电针组脊髓前角内浓染的标记细胞呈大多角状。电针组脊神经节及脊髓前角内标记细胞百分率及神经再生率明显高于对照组。上述结果表明,电针组坐骨神经干中感觉纤维及躯体运动纤维终末向各自支配区的再生速度明显快于对照组。本文结果提示:电针促进坐骨神经中不同纤维成分的再生可能具有定向诱导轴突支芽、加快神经细胞蛋白质合成及转运等双重功能。
In this experiment, the combination of flexor reflex-HRP retrograde tracing experiments was used to observe the effects of electro-acupuncture on somatosensory activity and the regeneration of sensory fibers in rat sciatic nerve trunk. The results showed that: 45 ± 5V flexor reflex threshold stimulation in the soles of the EA group caused a short latency incubation of Class A and long latency C fibers; in the control group, the sole was stimulated by 65 ± 5V flexor reflex threshold Only after the introduction of Class C fibers caused by flexor reflex. The right and left spinal ganglia in the electroacupuncture group were mostly large and small round cells were marked, while the control group of right ganglion labeled cells were lightly stained small round cells. Electroacupuncture spinal cord anterior horn stained cells were large-angle shape. The percentage of labeled cells and the rate of nerve regeneration in spinal ganglia and anterior horn of spinal cord in EA group were significantly higher than those in control group. The above results show that the regenerative speed of sensory fibers and somatofibric terminals in the sciatic nerve trunk of electroacupuncture group to their respective dominant areas is significantly faster than that of the control group. Our results suggest that electroacupuncture may promote the regeneration of different fiber components in the sciatic nerve with directional induction of axon branching and accelerate the dual functions of nerve cell protein synthesis and transport.