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目的研究积雪甙对黑素瘤细胞生长的影响。方法将黑素瘤细胞B16常规传代培养,取其生长状态良好的对数生长期细胞,观察积雪甙对B16细胞的生长抑制作用,并进行对B16细胞生长抑制作用的形态学观察,用流式细胞仪检测积雪甙对B16细胞诱导凋亡的作用。结果随着积雪甙的剂量增加,对B16细胞有丝分裂的抑制增加。代表凋亡早期细胞的annexin-v阳性细胞比例增加,摄取标记细胞线粒体的R123的细胞比例降低,而标记核内DNA的PI阳性细胞的比例增加,表明该药能够诱导细胞凋亡或致死亡。结论积雪甙有抗黑素瘤细胞生长作用。
Objective To study the effect of asiaticoside on the growth of melanoma cells. Methods Melanoma cells B16 were routinely subcultured and their growth logarithmic growth phase cells were obtained. The growth inhibition effect of asiaticoside on B16 cells was observed, and the morphological observation of the growth inhibition of B16 cells was performed. Effect of Asiaticoside on Induction of Apoptosis in B16 Cells by. Results As the dosage of asiaticoside increased, the inhibition of mitosis in B16 cells increased. The proportion of annexin-v positive cells that represent early apoptotic cells is increased, the proportion of cells that take up R123 in labeled mitochondria decreases, and the proportion of PI-positive cells that mark nuclear DNA increases, indicating that the drug induces apoptosis or death. Conclusion Asiaticoside has anti-melanoma cell growth.