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目的探讨血清脂联素水平及脂联素基因单核苷酸多态性(SNP)45T→G与2型糖尿病(T2DM)及代谢综合征(MS)的关系。方法采用放射免疫法对MS组患者183例、T2DM组患者180例和健康人群(对照组)144例进行血清脂联素水平测定,并用Taq Man探针技术进行脂联素基因SNP45分析。结果T2DM组和MS组血清脂联素水平较对照组显著降低(7.17±3.43)mg/L,(6.81±2.99)mg/L vs(12.06±5.74)mg/L(均P<0.05);多元逐步回归分析显示:空腹胰岛素是影响MS组患者血清脂联素水平的独立相关因素。脂联素基因多态性分析:SNP45在T2DM组、MS组与对照组比较差异有统计学意义(P<0.05);等位基因分布频率提示G等位基因在T2DM组与MS组中多见(P<0.05);T2DM组、MS组中携带TG+GG型的患者血清脂联素水平(6.63±2.79)mg/L、(6.19±2.40)mg/L显著低于TT型的患者(9.17±4.47)mg/L、(8.20±3.88)mg/L(P<0.05)。结论低脂联素血症与T2DM、MS的发生发展有关,SNP45 G/G基因型可能是中国汉族人MS的易感基因。
Objective To investigate the relationship between serum adiponectin level and adiponectin gene single nucleotide polymorphism (SNP) 45T → G and type 2 diabetes mellitus (T2DM) and metabolic syndrome (MS). Methods 183 cases of MS group, 180 cases of T2DM group and 144 cases of healthy people (control group) were measured by radioimmunoassay for serum adiponectin level. Adiponectin gene SNP45 was analyzed by Taq Man probe technique. Results Serum levels of adiponectin in T2DM group and MS group were significantly lower than those in control group (7.17 ± 3.43) mg / L, (6.81 ± 2.99) mg / L vs (12.06 ± 5.74) mg / L, respectively Stepwise regression analysis showed that fasting insulin was an independent factor influencing serum adiponectin levels in patients with MS. Adiponectin gene polymorphism analysis: There was a significant difference of SNP45 in T2DM group between MS group and control group (P <0.05). Allele distribution frequency suggested that G allele was more common in T2DM group and MS group (P <0.05). Serum levels of adiponectin in T2DM patients and MS patients with TG + GG were significantly lower than those with TT (6.63 ± 2.79) mg / L and (6.19 ± 2.40) mg / L, respectively ± 4.47) mg / L, (8.20 ± 3.88) mg / L respectively (P <0.05). Conclusion Low adiponectin is associated with the occurrence and development of T2DM and MS. SNP45 G / G genotype may be a susceptible gene to MS in Chinese Han population.