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目的探讨褪黑素受体1B基因(MTNR1B)多态性与青少年特发性脊柱侧凸(AIS)患者骨密度之间的相关性。方法选取511例年龄在12～18岁之间的女性AIS患者,采用双能X线骨密度吸收仪测量优势侧近端股骨的骨密度。同时,采取患者外周静脉血,采用试剂盒提取DNA。通过PCR-RFLP的方法检测MTNR1B基因启动子区上2个基因多态性位点的基因型。采用ANOVA的统计学方法比较不同基因型对应骨密度大小。结果MTNR1B基因2个多态性位点各基因型所对应的骨密度差异无显著性(P>0.05)。结论MTNR1B基因多态性与AIS患者的骨密度之间没有相关性,它可能在AIS患者的低骨量中不发挥作用。
Objective To investigate the association between melatonin receptor 1B gene (MTNR1B) polymorphism and bone mineral density in adolescent idiopathic scoliosis (AIS). Methods A total of 511 female AIS patients aged 12 to 18 years old were enrolled in this study. Bone mineral density (BMD) of proximal femur was measured by dual energy X-ray absorptiometry. At the same time, patients taking peripheral venous blood, using the kit to extract DNA. The genotypes of two gene polymorphisms in the promoter region of MTNR1B gene were detected by PCR-RFLP. Statistical methods of ANOVA were used to compare the BMD of different genotypes. Results There was no significant difference in BMD between the two genotypes of MTNR1B gene (P> 0.05). Conclusion There is no correlation between MTNR1B gene polymorphism and bone mineral density (BMD) in AIS patients. It may not play a role in the low bone mass of AIS patients.