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目的:采用不同的临床检测方法对小儿支原体肺炎患者的血清进行检测,并对其结果进行分析,探讨几种检验方法在小儿肺炎支原体感染的诊断过程中的意义以及价值。方法:对我院儿科2011年12月至2012年12月收治的肺炎支原体感染患儿135例的临床资料进行回顾性分析,分别采用超高倍显微镜法、微生物快速培养法(咽拭子)、肺炎支原体抗体检测(免疫)、PCR法和冷凝集试验方法进行临床检验。并采用统计学方法对各组实验数据进行分析。结果:135例受检样品中阳性结果分别为30例(65.2%)、39例(84.8%)、42例(91.3%)、18例(39%)、15例(32.6%)。135例呼吸道感染患儿符合临床诊断标准,诊断为MP感染的46例病例中,同时阳性15例;89例非MP感染组中,阳性者分别为2,7,5、3、3例。
OBJECTIVE: To detect the serum of children with mycoplasma pneumonia by different clinical tests and to analyze the results to explore the significance and value of several test methods in the diagnosis of children with Mycoplasma pneumoniae infection. Methods: The clinical data of 135 children with Mycoplasma pneumoniae infection from December 2011 to December 2012 in our hospital were analyzed retrospectively. The ultra-high magnification microscopy, rapid microbial culture (throat swab), pneumonia Mycoplasma antibody test (immunization), PCR method and condensation test method for clinical testing. And statistical methods were used to analyze the experimental data of each group. Results: The positive results of 135 samples were 30 cases (65.2%), 39 cases (84.8%), 42 cases (91.3%), 18 cases (39%) and 15 cases (32.6%). 135 cases of respiratory tract infection in children meet the clinical diagnostic criteria, the diagnosis of MP infection in 46 cases, while 15 cases were positive; 89 cases of non-MP infection, positive were 2,7,5,3,3 cases.