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【目的】研究生后早期长期反复母婴分离对子代大鼠成年后海马星形胶质胶质细胞的影响。【方法】新生大鼠分为母婴分离组和对照组,每组分别有雄性和雌性大鼠各10只。母婴分离组大鼠于生后第2 d至生后第14 d每日与母鼠分离3 h,对照组大鼠不受干扰。各组大鼠于生后第60 d处死,采用免疫组化方法分析各组大鼠背侧和腹侧海马星形胶质细胞标记物(GFAP和S100β)的表达。【结果】双因素方差分析显示:与对照组相比,母婴分离组雄性大鼠成年后腹侧海马GFAP和S100β染色阳性细胞数显著减少(P<0.05);背侧海马星形胶质细胞中GFAP和S100β平均光密度明显下降(P<0.001)。【结论】母婴分离可使子代雄性大鼠成年后腹侧海马星形胶质细胞数量减少,背侧海马星形胶质细胞中GFAP和S100β含量下降。
【Objective】 To study the effects of long-term repeated maternal-neonatal separation on hippocampal astrocytes in offspring of offspring. 【Methods】 Newborn rats were divided into maternal and child separation group and control group. There were 10 male and female rats in each group. The maternal and child separation rats were separated from their females for 3 hours on the 2nd day after birth and on the 14th day after birth, and the rats in the control group were not disturbed. The rats in each group were sacrificed on the 60th day after birth, and the expression of GFAP and S100β in the dorsal and ventral hippocampal astrocytes was detected by immunohistochemistry. 【Results】 Two-factor analysis of variance showed that compared with the control group, the number of GFAP and S100β-positive cells in the ventral hippocampus of maternal and neonatal rats was significantly decreased (P <0.05); the dorsal hippocampal astrocytes The average optical density of GFAP and S100β was significantly decreased (P <0.001). 【Conclusion】 Maternal and fetal separation can decrease the number of ventral hippocampal astrocytes and the decrease of GFAP and S100β in dorsal hippocampal astrocytes.