论文部分内容阅读
目的 为开展人卡氏肺孢子虫的病原学形态观察、免疫化学、分子生物学特性以及药物治疗的研究建立卡氏肺孢子虫肺炎大鼠模型。方法 给 SD大鼠腹股沟皮下注射醋酸可的松 ,6～12周后 ,收集支气管肺泡灌洗液和制作肺组织印片 ,组化染色 ,镜下观察。结果 实验大鼠的肺印片肺孢子虫病原学染色阳性率为 96 % (2 4/ 2 5 ) ,在 2 4只肺孢子虫病原学染色检查阳性的大鼠中 ,11只大鼠 BAL s沉淀涂片阳性。本次诱导实验包囊检出时间最早的为用药 40天 ,且随用药时间的延长 ,感染程度逐渐加重。对照组均未检出阳性。结论 建立了卡氏肺孢子虫肺炎大鼠模型。
OBJECTIVE To develop etiological morphological observation, immunochemistry, molecular biology and drug treatment of Pneumocystis carinii in rats, a rat model of Pneumocystis carinii pneumonia was established. Methods Cortisone acetate was injected subcutaneously into the groin of SD rats. After 6 to 12 weeks, bronchoalveolar lavage fluid was collected and printed on the lung tissue. Staining and microscopic observation were performed. Results The positive rate of pathogenic Pneumocystis encephalitis was 96% (2 4/2 5) in lungs of experimental rats. Of the 24 rats positive for pathogenic Pneumocystis, 11 rats BAL spositive smear positive. The induction of cysts detected the earliest time for 40 days, and with the extension of medication time, the degree of infection gradually aggravated. The control group were not detected positive. Conclusion The model of Pneumocystis carinii pneumonia was established in rats.