Objective: To investigate the relation of transfergrowth factor (TGF-β_1) and β-glucuronidase (β-GCD) on the occurrence and progress of pancreaticcancer.Methods: The expression of TGF-β_1 and β-GCD in thepancreatic cancer tissue and normal pancreatic tissuewas determined synchronously using ABC method ofimmunohistochemistry.Results: The percentage of TGF-β_1 positive cells wassignificantly higher in pancreatic cancer tissue (43.8%±5.2%) than in adjacent pancreatic tissue (28.7%±3.6%, P<0.01). The worse the cancer cells differen-tiated and lymph nodes metastasis, the more over-ex-pression of TGF-β_1. The percentage of β-GCD positivecells was also significantly higher in the pancreaticcancer tissue (62.5%±4.1%) than in the adjacentpancreatic tissue (33.5%±2.8%, P<0.01). The de-gree of over-expression of β-GCD was related to thedegree of cancer cells differentiation, but not to thelymph nodes metastasis. The expression of TGF-β_1was significantly correlated with the expression of β-GCD in pancreatic cancer tissue.Conclusions: The genesis of pancreatic cancer resultsfrom multi-factor, multi-step and multi-gene varia-tion. The synchronous detection of TGF-β_1 and β-GCD helps to determine the malignant degree oftumors and the prognosis of patients with such disease. Objective: To investigate the relation of transfer of growth factor (TGF-β_1) and β-glucuronidase (β-GCD) on the occurrence and progress of pancreatic cancer. Methods: The expression of TGF-β_1 and β-GCD in the pancreatic cancer tissue and normal pancreatic Results: The percentage of TGF-β_1 positive cells wassignificantly higher in pancreatic cancer tissue (43.8% ± 5.2%) than in adjacent pancreatic tissue (28.7% ± 3.6%, P <0.01). The worse the percentage of β-GCD positive cells was also significantly higher in the pancreatic cancer tissue (62.5% ± 4.1%) than in the adjacent pancreatic tissue (33.5% ± 2.8%, P <0.01). The de-gree of over-expression of β-GCD was related to the degree of cancer cells differentiation, but not to the lymph nodes metastasis. The expression of TGF-β_1 was significantly correlated with the expressi on of β-GCD in pancreatic cancer tissue. Conclusions: The genesis of pancreatic cancer resultsfrom multi-factor, multi-step and multi-gene varia tion. The synchronous detection of TGF-β_1 and β-GCD helps determine the malignant degree oftumors and the prognosis of patients with such disease.