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目的： 观察蓖麻毒素（ＲＴ）修饰前后对小鼠肝脏毒性的影响． 方法： ＲＴ用３－（２－吡啶二巯基）丙酸Ｎ－羟基琥珀酰亚胺酯（ＳＰＤＰ） 一种异型双功能交联剂，进行化学修饰；生成ＲＴ的ＳＰＤＰ衍生物（ＰＤＰ－Ｒ），测定两者在不同剂量和时间对小鼠肝脏还原型谷胱甘肽（ＧＳＨ）含量的影响，以比较两者的毒性差别． 结果： 随着中毒剂量的增加和时间的延长，两者均可使小鼠肝脏ＧＳＨ含量下降（Ｐ＜ ０．０１），但ＲＴ组下降程度较ＰＤＰ－Ｒ组更加明显（Ｐ＜ ０．０１），表明ＰＤＰ－Ｒ对小鼠毒性弱于ＲＴ． 结论： 用ＳＰＤＰ修饰ＲＴ可降低其对小鼠的毒性，这对改进ＲＴ抗癌作用可能是一种有意义的尝试．
Objective: To observe the effect of ricin (RT) modification on mouse liver toxicity before and after modification. METHODS: RT was modified by chemical modification of a heterobifunctional bifunctional crosslinker with 3- (2-pyridyldithiol) propionic acid N-hydroxysuccinimide ester (SPDP). The RT-producing SPDP derivative (PDP-R) , To determine the effect of both on the content of hepatic reduced glutathione (GSH) at different dosage and time in order to compare the difference of the two. Results: With the increase of poisoning dose and prolongation of time, both of them decreased the content of GSH in liver of mice (P <0.01), but decreased more significantly in RT group than in PDP-R group (P <0.01) ), Indicating that the toxicity of PDP-R to mice is weaker than that of RT. Conclusion: Modification of RT with SPDP can reduce its toxicity to mice, which may be a meaningful attempt to improve the anticancer effect of RT.